A micromethod for the routine estimation of inorganic phosphate in serum and urine.

Relation between manual and automatic values nine serum samples by both the classical manual method of WARREN and by the proposed automatic method. The results are shown in table 1 and figure 5. As can be seen, the Autoanalyzer method gives consistantly higher results by about 8%. This is most probably due to the incompleteness of the extraction of the coloured end product in the manual method. Finally, we checked the effect of the most probable source of interference, namely the blood glucose. No interference could be detected by the addition of up to 10 times the normal amount of glucose. This method has been in daily use in our laboratory for over a year and has been entirely satisfactory.